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| 2. |  | ALMEIDA, V. M.; LUZ, G. A.; MARTINS, P. P.; GOMES, M. F. C.; COSTA, M. F.; LIMA, P. S. da C.; VALENTE, S. E. S. Comparison of eight methods to isolate genomic DNA from Hancornia speciosa. Genetics and Molecular Research, Ribeirão Preto, v.16, n. 3: gmr16039724, 2017. 7 p.| Biblioteca(s): Embrapa Meio-Norte. |
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| 3. | | SANTOS, J. A.; LUZ, G. A.; OLIVEIRA, K. P.; OLIVEIRA, L. F.; ANDRADE JUNIOR, A. S. de; VALENTE, S. E. S.; LIMA, P. S. da C. DNase concentration assay to obtain DNA-free RNA from sugarcane leaves. Genetics and Molecular Research, v. 15, n. 4, p. 1-12, 2016.| Biblioteca(s): Embrapa Meio-Norte. |
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| 4. | | LUZ, G. A.; GOMES, S. O.; ARAUJO NETO, R. B. de; NASCIMENTO, M. S. C. B.; LIMA, P. S. da C. Molecular characterization of accessions of Cratylia argentea (Camaratuba) using ISSR markers. Genetics and Molecular Research, Ribeirão Preto, v. 14, n. 4, p. 15242-15248, nov. 2015.| Biblioteca(s): Embrapa Meio-Norte. |
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| Registros recuperados : 4 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Meio-Norte. |
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Data corrente: |
01/12/2015 |
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Data da última atualização: |
24/05/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
LUZ, G. A.; GOMES, S. O.; ARAUJO NETO, R. B. de; NASCIMENTO, M. S. C. B.; LIMA, P. S. da C. |
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Afiliação: |
G.A. Luz, Laboratório de Biologia Molecular, Centro de Ciências Agrárias, Universidade Federal do Piauí, Teresina, PI.; S.O. Gomes, Laboratório de Biologia Molecular, Centro de Ciências Agrárias, Universidade Federal do Piauí, Teresina, PI.; RAIMUNDO BEZERRA DE ARAUJO NETO, CPAMN; M.S.C.B. Nascimento, APOSENTADA; PAULO SARMANHO DA COSTA LIMA, CPAMN. |
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Título: |
Molecular characterization of accessions of Cratylia argentea (Camaratuba) using ISSR markers. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Genetics and Molecular Research, Ribeirão Preto, v. 14, n. 4, p. 15242-15248, nov. 2015. |
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ISSN: |
1676-5680 |
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DOI: |
http://dx.doi.org/10.4238/2015.November.25.12 |
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Idioma: |
Inglês |
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Conteúdo: |
Cratylia argentea (Desv.) Kuntze (Fabaceae) is a drought-tolerant, perennial legume found primarily in Brazil, Bolivia, and Peru. The shrub is well adapted to acid soils and exhibits high productivity and nutritional value, characteristics that would favor its use as a dry season animal forage supplement in semiarid regions. In plant improvement programs, the production of elite hybrids with superior traits is generally achieved by crossing parents that exhibit the highest level of genetic divergence. Therefore, the aim of the present study was to assess genetic diversity among 13 accessions of C. argentea from the same population maintained in the active germplasm bank of Embrapa Meio-Norte using inter-simple sequence repeat (ISSR) markers. Genetic similarities between C. argentea accessions were estimated from Jaccard coefficients, and a dendrogram was constructed using the unweighted pair group method with arithmetic average (UPGMA). The set of 15 primers selected for ISSR analysis generated a total of 313 loci of which 79.23% were polymorphic. The mean number of bands per primer was 20.87, and the amplicons ranged from 280 to 3000 bp in size. Primers UBC834 and UBC827 generated the largest number of polymorphic loci and exhibited 90.91 and 100% polymorphism, respectively. The coefficients of genetic similarity among accessions varied between 0.49 and 0.73. UPGMA cluster analysis allowed the identification of four genotypic groups and demonstrated the existence of considerable variability within the collection. Potential progenitors were selected that would offer good possibilities of obtaining unusual and favorable combinations of genes in a plant breeding program. MenosCratylia argentea (Desv.) Kuntze (Fabaceae) is a drought-tolerant, perennial legume found primarily in Brazil, Bolivia, and Peru. The shrub is well adapted to acid soils and exhibits high productivity and nutritional value, characteristics that would favor its use as a dry season animal forage supplement in semiarid regions. In plant improvement programs, the production of elite hybrids with superior traits is generally achieved by crossing parents that exhibit the highest level of genetic divergence. Therefore, the aim of the present study was to assess genetic diversity among 13 accessions of C. argentea from the same population maintained in the active germplasm bank of Embrapa Meio-Norte using inter-simple sequence repeat (ISSR) markers. Genetic similarities between C. argentea accessions were estimated from Jaccard coefficients, and a dendrogram was constructed using the unweighted pair group method with arithmetic average (UPGMA). The set of 15 primers selected for ISSR analysis generated a total of 313 loci of which 79.23% were polymorphic. The mean number of bands per primer was 20.87, and the amplicons ranged from 280 to 3000 bp in size. Primers UBC834 and UBC827 generated the largest number of polymorphic loci and exhibited 90.91 and 100% polymorphism, respectively. The coefficients of genetic similarity among accessions varied between 0.49 and 0.73. UPGMA cluster analysis allowed the identification of four genotypic groups and demonstrated the existence of conside... Mostrar Tudo |
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Palavras-Chave: |
Caracterização molecular; Diversidade genética; Forage crop; Genetic diversity; ISSR markers; Molecular characterization. |
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Thesagro: |
Marcador molecular; Planta forrageira. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/134533/1/ArtigoSarmanhoGMR6985cam.pdf
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Marc: |
LEADER 02619naa a2200289 a 4500
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022 $a1676-5680
024 7 $ahttp://dx.doi.org/10.4238/2015.November.25.12$2DOI
100 1 $aLUZ, G. A.
245 $aMolecular characterization of accessions of Cratylia argentea (Camaratuba) using ISSR markers.
260 $c2015
520 $aCratylia argentea (Desv.) Kuntze (Fabaceae) is a drought-tolerant, perennial legume found primarily in Brazil, Bolivia, and Peru. The shrub is well adapted to acid soils and exhibits high productivity and nutritional value, characteristics that would favor its use as a dry season animal forage supplement in semiarid regions. In plant improvement programs, the production of elite hybrids with superior traits is generally achieved by crossing parents that exhibit the highest level of genetic divergence. Therefore, the aim of the present study was to assess genetic diversity among 13 accessions of C. argentea from the same population maintained in the active germplasm bank of Embrapa Meio-Norte using inter-simple sequence repeat (ISSR) markers. Genetic similarities between C. argentea accessions were estimated from Jaccard coefficients, and a dendrogram was constructed using the unweighted pair group method with arithmetic average (UPGMA). The set of 15 primers selected for ISSR analysis generated a total of 313 loci of which 79.23% were polymorphic. The mean number of bands per primer was 20.87, and the amplicons ranged from 280 to 3000 bp in size. Primers UBC834 and UBC827 generated the largest number of polymorphic loci and exhibited 90.91 and 100% polymorphism, respectively. The coefficients of genetic similarity among accessions varied between 0.49 and 0.73. UPGMA cluster analysis allowed the identification of four genotypic groups and demonstrated the existence of considerable variability within the collection. Potential progenitors were selected that would offer good possibilities of obtaining unusual and favorable combinations of genes in a plant breeding program.
650 $aMarcador molecular
650 $aPlanta forrageira
653 $aCaracterização molecular
653 $aDiversidade genética
653 $aForage crop
653 $aGenetic diversity
653 $aISSR markers
653 $aMolecular characterization
700 1 $aGOMES, S. O.
700 1 $aARAUJO NETO, R. B. de
700 1 $aNASCIMENTO, M. S. C. B.
700 1 $aLIMA, P. S. da C.
773 $tGenetics and Molecular Research, Ribeirão Preto$gv. 14, n. 4, p. 15242-15248, nov. 2015.
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Embrapa Meio-Norte (CPAMN) |
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